Beckman Coulter EPICS Elite ESP
Location: Medical Sciences Building, E-25
|UIC Cost||$NA (per hour)||$80 (per hour)|
|Other Academic||$NA (per hour)||$124 (per hour)|
|Non-Academic;||$NA (per hour)||$248 (per hour)|
The EPICS Elite ESP is the latest generation of the Elite product line. This high-performance instrument is a powerful tool for innovative research in immunobiology, cell physiology, molecular biology, genetics, water quality and plant cell physiology. The Elite ESP - Enhanced System Performance - features the capability to analyze up to 4 simultaneous colors of immunofluorescence and perform equally complex multi-parameter applications such as DNA quantitation, physiologic measurements, chromosome enumeration and the study of the hematopoetic process. The cell sorting capability of the system allows for the rapid separation of very large numbers of specific cell populations from a heterogeneous mixture. Extremely high purities -greater than 99 percent - are achieved, even at high rates of analysis.
Dual Excitation Sources: 1. 15mW air cooled Argon Ion Laser at 488nm: Used to excite Fluoroscein (FITC), Phycoerythrin (PE), PE-Cy5 (Cychrome/Tricolor), PE-Texas Red (ECD/Red 613), Red 670, Rhodamine 123, Propidium Iodide (PI), Acridine Orange, and other compounds. 2. 10mW air cooled Helium-Neon Laser at 633nm (red): Used to excite Allophycocyanin (APC) and Cy5. If APC or Cy5 is used, they should be bright compared to your other reagents in a multicolor experiment. Helpful suggestions: If cells are to be analyzed on this instrument, it is best that the concentration of cells/particles is 1-5 million cells/ml. Sorting concentrations may be higher especially when the enrichment of rare cells/particles is desired. The cells should be placed in 12 x 75 mm tubes (minimum volume of 250 µl). Sorted cells can be deposited into either 15 ml conical centrifuge tubes or 12 x 75 mm tubes. However, it is easier to sort into the smaller tubes. These tubes should be 75% filled with medium or PBS containing high amounts of protein (i.e. fetal calf serum, bovine serum albumin, etc.).