Applied Biosystems, Inc. 4700 Proteomics Analyzer
Location: Science and Engineering South, 109
|UIC Cost||$25 (per hour)||$52 (per hour)|
|Other Academic||$52 (per hour)||$81 (per hour)|
|Non-Academic;||$104 (per hour)||$162 (per hour)|
The Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF™ Optics acquires MS and MS/MS spectra in a high-throughput, easy-to-use MALDI platform. It supports a wide variety of front-end sample preparation methods, such as 1-D and 2-D gels, multi-dimensional liquid chromatography (MDLC), or Applied Biosystems proprietary Isotope Coded Affinity Tag technology, as well as back-end bioinformatics platforms. The 4700 Proteomics Analyzer has a coaxial tandem time-of-flight MS/MS configuration providing high-energy collision-induced dissociation for fragmentation with structural information such as immonium ions, side-chain cleavages, and internal fragments. This structural detail can elucidate posttranslational modifications and leucine/isoleucine differentiation and other complexities within high-resolution proteomics. The large mass range and high-energy fragmentation of the TOF/TOF™ optics can analyze proteins without prior digestion. The high rep-rate laser and high-speed data acquisition system provide high sensitivity and dynamic range.
The Applied Biosystems 4700 Proteomics Analyzer also includes a 200 Hz laser and acquisition system.