Beckman Coulter Cyan ADP
Location: Medical Sciences Building, E-25
|UIC Cost||$39 (per hour)||$69 (per hour)|
|Other Academic||$60.45 (per hour)||$106.95 (per hour)|
|Non-Academic;||$120.9 (per hour)||$213.9 (per hour)|
There are two Cyan ADP analysers, one located at E-25, MSB and the other at E-817 MSB. The CyAn ADP Analyzer is the next step in Advanced Digital Processing with: * Enhanced detection for flow cytometry * State-of-the-art optics and electronics * Automated compensation * Easy to walk-up operation. CyAn ADP 9 Color adds the 405nm solid state laser and two fluorescent channels. Additional research applications include stem cell/SP analysis, DAPI Cell Cycle analysis and CFP-YFP FRET analysis. This Cyan1 is available only for self-run by investigators who have been trained by the flow cytometry core lab faculty. The Cyan2 is available for self-run users AND for investigators who need assisted acquistion of samples. First preference will be given to users who require assisted acquisition. Biohazardous samples must not be run on the CyAn. Any potentially biohazardous sample must be fixed. The instrument is located at E-25 (in the basement), Medical Sciences building, 835 S Wolcott Avenue, Chicago, IL 60612.
Laser Light Source Parameters Fluorochromes 488nm blue laser)- FL1- FITC, GFP, CFSE, Alexa Fluor 488, YFP; FL2-PE; FL3PE-Alexa Fluor610, PI, Qdot605; FL4- PC5, PerCP, PC5.5, PerCP-Cy5.5, 7-AAD, Qdot655; FL5- PC7, PE-Alexa Fluor 750, Qdot705 405nm (violet laser) FL6- Pacific Blue, Cascade Blue, DAPI, CFP; FL7- Cascade yellow,Pacific Orange✝, AmCyan*, Qdot565* 642 nm (red laser) FL8-APC, AF647; FL9- APC-Cy7, APC-ef780,Qdot800