Olympus VivaView FL

Olympus VivaView FL

Location: Medical Sciences Building, E-331

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  Unassisted Assisted
(1 hr)
UIC Cost $20 (< 4 hr), then $6 (per hour) $20 (< 4 hr), then $6 (per hour)
Other Academic $31 (< 4 hr), then $9 (per hour) $31 (< 4 hr), then $9 (per hour)
Non-Academic$40 (< 4 hr), then $1 (per hour) $40 (< 4 hr), then $1 (per hour)

Description

Just as its name implies, the Olympus VivaView FL incubator/fluorescence microscope consists of an inverted fluorescent microscope that is fully contained in a moderate-sized CO2 incubator. Such a configuration allows for extremely stable time lapse imaging in an environment that is identical to the most common in vitro cell culture conditions. The system also features a motorized stage for monitoring multiple positions in a dish, as well as an automated tray with space for up to 8 dishes. Consequently, whole experiments and a full set of controls can be precisely imaged in parallel for as long as biologically feasible - the final image number/file size is generally the only practical consideration.

Such long-term stability does come at an optical expense. The VivaView is limited to a single 40x objective (40x/0.95 NA), which can be further adjusted (with no gain in resolution) to either 20x or 80x via additional lenses. The objective is, however, well-suited for DIC (differential interference contrast) imaging, and produces transmitted light images of excellent quality. Basic fluorescent capture is also possible through a 200W mercury arc lamp X-Cite eXacte illumination system and a set of FITC (green) and TRITC (red) filter sets.

Transmitted light can become birefringent when passed through polystyrene (plastic) cultures dishes/lids. As a result, effective DIC imaging in the VivaView requires that cells be plated in certain round 35 mm glass-bottom dishes, which are available for purchase from the facility. In addition, the plastic culture dish lids must be replaced with specialized glass covers that are maintained by the facility staff. (Note that these lids are not disposable, and must be kept with the instrument at all times - they are merely swapped with incoming samples' lids immediately prior to imaging.)

The VivaView is controlled via a PC workstation running an aquisition version of the MetaMorph software. The microscope control interface is straightforward and intuitive, but the software itself does hide a number of complex tools that may not be required by most users. Capture is performed with an ORCA-R2 Hamamatsu cooled CCD camera (1344 x 1024 pixels).

In all, the VivaView has the potential to streamline several common imaging approaches, including wound healing/scratch assays, timed drug delivery, migration studies, and observations of fluorescent protein dynamics.

More details can be found at the RRC Wiki guide for this instrument.

Technical Specifications

Some of the highlighted specifications include:
• UPLSAP40x objective (NA 0.95, WD 0.18 mm)
• 0.5x/1x/2x intermediate magnifications
• 1W LED for transmitted light observation via DIC
• x-Cite eXacte DC lamp for fluorescence observation
• 35 mm dish sample capacity, up to 8 total
• Objective drive focus control
• -35° air cooled CCD camera, 12 or 16 bits
• MetaMorph imaging software