Carl Zeiss LSM 710
Location: Medical ScienceS Building, E-330
|UIC Cost||$41 (per hour)||$76 (per hour)|
|Other Academic||$64 (per hour)||$99 (per hour)|
|Non-Academic;||$82 (per hour)||$117 (per hour)|
The Zeiss LSM 710 confocal microscope is a powerful point-scanning system that streamlines many steps of the configuration and acquisition process. While the capabilities of the LSM 710 are similar to those found on the widely-used LSM 510 META, the LSM 710 captures reflected light using a fully adjustable prism instead of an arrangement of preset emission filters. The result is greater control of fluorescent imaging and significantly higher sensitivity.
The LSM 710 is controlled via the ZEN software utility. The current version boasts a number of processing improvements, such as clearer colocalization readouts, built-in FRAP normalization controls, and enhanced spectral unmixing tools. Features available in earlier software versions, including ratiometric imaging, time series recording, and 3D capture/rendering, are also present and upgraded.
One of the most welcome new features of the ZEN software is the "Smart Setup" option, which demystifies many of the configuration steps that are required for older Zeiss instruments. Upon clicking the "Smart Setup" button, users are guided through a color selection process among a reference library that contains hundreds of common fluorophores. After inputing the experiment details, an investigator simply selects from the recommended configurations to optimize image quality and/or capture time.
Also available is the optional Definite Focus module, an infrared light-controlled system that operates independently and automatically to correct deviations from an initial focus setting. The Definite Focus component is housed separately from the microscope scanner, but can be activated through either the touchscreen display or within the ZEN software.
In addition, a PeCon Tempcontrol 37-2 temperature regulator is housed near the instrument, which is connected to an aluminum heating insert. When mounted on the LSM 710 microscope stage, the insert provides an adjustable conductive heat source. Live cells in 35 mm culture dishes can thus be maintained for longer time course experments.
More details can be found at the RRC Wiki guide for this instrument.
Available excitation lasers include:
• 30 mW diode UV laser = 405 nm (DAPI, Hoechst)
• 25 mW Multi-line Ar laser = 458, 488, 514 nm (458 for CFP; 488 for GFP, CY2, FITC; 514 for YFP)
• 20 mW solid-state diode laser = 561 nm (Rhodamine, CY3, Propidium Iodide, Alexa 568)
• 5 mW HeNe laser = 633 nm (deep red, CY5)
Currently configured objectives include:
• 10x/0.25 Ph1 A-Plan
• 25x/0.8 Multi Immersion DIC Plan-Neofluar
• 40x/1.2 Water DIC C-Apochromat
• 63x/1.2 Water DIC C-Apochromat
• 63x/1.46 Oil alpha Plan-Apochromat
Bandpass sliders in front of 2 fixed spectral detectors and an additional 32-channel detector allow for emission bands to be precisely selected without separate filters or secondary dichroics. Emission wavelengths between ~400 and 750 nm can be detected.